|
KMID : 1101420170490010028
|
|
Korean Journal of Clinical Laboratory Science
2017 Volume.49 No. 1 p.28 ~ p.39
|
|
|
Global DNA Methylation Patterns and Gene Expression Associated with Obesity-Susceptibility in Offspring of Pregnant Sprague-Dawley Rats Exposed to BDE-47 and BDE-209
|
|
Park Byeong-Min
Yoon Ok-JIn
Lee Do-Hoon
|
|
|
Abstract
|
|
|
|
Persistent organic pollutants (POPs) can affect epigenetic mechanisms and obesity development. Polybrominated diphenyl ethers (PBDEs)?widely used to make flames?are one of the important POPs. Prenatal exposure to endocrine disrupting chemicals (EDCs), such as POPs, may affect global DNA methylation in long interspersed nuclear elements (LINE-1), increasing the risk of obesity later in life. Therefore, pregnant Sprague-Dawley (SD) rats were used to elucidate whether BDE-47 and BDE-209 transferred through placenta and breast milk cause epigenetic changes in LINE-1 and increase genetic susceptibility to obesity as obesogen during the developmental periods. Global DNA methylation in LINE-1 and gene expression related to obesity were measured in dams and offspring, using a methylation-sensitive high resolution melting analysis (MS-HRM) and direct bisulfite sequencing and quantitative real time polymerase chain reaction (qPCR), respectively. The results of MS-HRM showed global DNA hypomethylation patterns in LINE-1 of exposed offspring (2 of total 4) at PND 4, but bisulfite sequencing showed no difference in both the exposed and non-exposed groups. Gene expression in dams related to β-oxidation pathway and those related to adipokines showed different patterns between the two groups. On the contrary, gene expressions of offspring showed a similar pattern. Gene expressions related to β-oxidation pathway and obesity were significantly increased when compared with ’at birth’, but not PPAR-α. In conclusion, this study demonstrated the possibility that co-exposure to BDE-47 and BDE-209?via the placenta and breast milk?may affect epigenetic changes and modulate gene expression levels related to obesity.
|
|
|
KEYWORD
|
|
|
Polybrominated diphenyl ethers, Epigenetics, DNA methylation, Obesity, Beta-oxidation, Adipokines
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|